The Rate of Uptake of Carbon Monoxide and of Nitric Oxide by Normal Human Erythrocytes and Experimentally Produced Spherocytes

نویسندگان

  • Elizabeth Carlsen
  • Julius H. Comroe
چکیده

THE UPTAKE OF GASES SUCH AS OXYGEN, CARBON MONOXIDE, OR NITRIC OXIDE BY THE ERYTHROCYTE INVOLVES: (a) diffusion across the cellular membrane, (b) intraerythrocytic diffusion, and (c) chemical combination with hemoglobin. The aim of this investigation was to obtain data which would permit an analysis of each of these factors in limiting the rate of gas uptake. The initial over-all rate of uptake of gases which combine chemically with hemoglobin to produce a color change can be measured by a modified version of the Hartridge-Roughton-Millikan constant flow, rapid reaction apparatus. If nitric oxide is the reactant gas, only (a) and (b) are measured since the chemical combination of this gas with hemoglobin is extremely rapid. Our studies have shown that human biconcave discoidal erythrocytes at 38 and 48 degrees C., have the same initial rate of carbon monoxide and nitric oxide uptake as the same cells converted into spherocytes of equal volume. Similarly there was no difference between discs and cells sphered with a 30 per cent increase in volume. Shrunken erythrocytes showed a marked decrease in rate of gas uptake. This suggests that surface area and maximum linear distance for intracellular diffusion of this magnitude do not measurably retard gas uptake. In the shrunken cells, a change in the orientation and concentration of intraerythrocytic hemoglobin and/or of the membrane components may have impeded gas diffusion.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Rate of Uptake of Carbon Monoxide and of N I T R I C O X I D E by Normal Human Erythrocytes

The uptake of gases such as oxygen, carbon monoxide, or nitric oxide by the erythrocyte involves: (a) diffusion across the cellular membrane, (b) intraerythrocytic diffusion, and (c) chemical combination with hemoglobin. The aim of this investigation was to obtain data which would permit an analysis of each of these factors in limiting the rate of gas uptake. The initial over-aU rate of uptake ...

متن کامل

Doxycycline in lymphangioleiomyomatosis: not all questions are answered.

1 Glénet SN, De Bisschop C, Vargas F, et al. Deciphering the nitric oxide to carbon monoxide lung transfer ratio: physiological implications. J Physiol 2007; 582: 767–775. 2 Hughes JM, van der Lee I. The TL,NO/TL,CO ratio in pulmonary function test interpretation. Eur Respir J 2013; 41: 453–461. 3 Guenard H, Varene N, Vaida P. Determination of lung capillary blood volume and membrane diffusing ...

متن کامل

Catalytic Oxidation of Carbon Monoxide by Cobalt Oxide Catalysts Supported on Oxidized-MWCNT

Cobalt oxide catalysts supported on oxidized multi-walled carbon nanotubes (MWCNT) for the low-temperature catalytic oxidation of carbon monoxide were prepared by an impregnation-ultrasound method. These catalysts were characterized by N2 adsorption/desorption, TEM, XRD, Raman, and H2-TPR methods. The XRD and Raman results indicated that the phase of the synthesized cobalt...

متن کامل

Developed Method Application for Nitrite Ion (NO2¯ ) Analysis of Tib -186 Macrophage Like Cell Lines by Rapid Isocratic HPLC System with High Sensitive Glassy Carbon Electrochemical Detector

A rapid isocratic method of high performance liquid chromatography system (HPLC) with a glassy carbon working electrode of electrochemical detector is set up for quantitative detection of  trace amount of nitrite ion (NO2¯) in aqueous protein containing cell lysate, cell media, plasma, serum, urine and other body fluids. The solid extraction  reversed phase cartridges ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • The Journal of General Physiology

دوره 42  شماره 

صفحات  -

تاریخ انتشار 1958